Heterogeneous Cytoskeletal Force Distribution Delineates the Onset Ca2+ Influx Under Fluid Shear Stress in Astrocytes
Mechanical perturbations increase intracellular Ca2+ in cells, but the coupling of mechanical forces to the Ca2+ influx is not well understood.We used a microfluidic chamber driven with a high-speed pressure servo to generate defined fluid shear stress to cultured HEART FORMULA astrocytes, and simultaneously measured cytoskeletal forces using a for